The museum’s collection comprises objects relating to the history of the Berrima District and Wingecarribee Shire.  It should be noted that not all items in the collection are on display at any one time. Significant items in the collection include:

• Fine lace and linen
• Period costumes and wearing apparel, for both men and women, dating back to the 1880s
   
•  Dr George Prowd Lambert collection. This includes his scribbling diaries for the years 1878 and 1882, some of his surgical implements, the brass shingle from his surgery door and an original photograph of Dr Lambert in a handsome frame.
   
• Small hand-made soft dolls, dressed in English period costumes, by local identity Margaret Kyngdon
   
• Country kitchen paraphernalia from the property 'Roberton Park', at Glenquarry
   
• Small military items, trench art and uniforms
   
• A dainty cap with a gum leaf motif and a metal gum-leaf shaped badge. These were worn by members of the unique local Blue Gum Girls. They served refreshments at Moss Vale railway station to troops en-route to WW1 and WW11.